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Supplemental Fig. 2. Wnt/β-catenin signaling regulates Mespo expression. (A, B) Enhancing Wnt/β-catenin signaling activity increases Mespo expression. Four-cell-stage embryos were injected in VMZ of one cell with pCSacZ (A) or together with pCS-Beta-catenin DNA (B). While the expression of Mespo in the pCSacZ injected side in stage 19 embryos is the same as the uninjected side (A), the expression of Mespo in the pCS-beta-catenin injected side is increased (B). Arrowhead in (B) indicates the increase of Mespo expression and arrow indicates the expression of Mespo in the uninjected side. For all embryos, anterior is to the left. (C, D) Overexpression of Lef1-EnR mRNA decreases the expression of Mespo. Four-cell-stage embryos were injected in VMZ with 50 pg LacZ mRNA (C) or together with 400 pg Lef1nR mRNA (D). (C) Expression of Mespo at stage 10. No significant difference can be found between the uninjected side and injected side. (D) In Lef1nR mRNA injected side, Mespo expression is absent (80%, N = 50). Arrowhead in (D) indicates the decrease of Mespo expression. All the embryos are vegetal view with dorsal up. |