|
| Gene |
Clone |
Species |
Stages |
Anatomy |
|
msgn1.L
|
|
laevis
|
NF stage 10.5
to
NF stage 11.5
|
mesoderm
,
ventral marginal zone
,
ventro-lateral marginal zone
,
dorso-lateral marginal zone
,
involuting marginal zone
|
|
msgn1.L
|
|
laevis
|
NF stage 18
to
NF stage 25
|
mesoderm
,
circumblastoporal collar
,
posterior
|
|
msgn1.L
|
|
laevis
|
NF stage 26
|
head region
,
tail bud
|
|
msgn1.L
|
|
laevis
|
NF stage 29 and 30
|
head region
,
post-anal gut
,
tail bud
|
|
msgn1.L
|
|
laevis
|
NF stage 35 and 36
|
head region
,
chordoneural hinge
,
periocular region
|
|
| |
Fig. 3. Whole-mount in situ hybridization analysis of Mespo expression. (1) A stage 10.5, vegetal view, shows Mespo expression in the ventral and lateral
mesoderm. Note that expression is excluded from the region above the dorsal blastopore lip. (2) Stage 11. (3) Stage 11.5. Expression is seen in the lateral
mesoderm extending away from the blastopore lip. (4) Stage 18, a lateral view with anterior oriented towards the left and posterior towards the right, with
dorsal at the top and ventral at the bottom. Strong expression is evident in the tailbud and weak expression in the lateral mesoderm. (5) Stage 20. (6) Stage 25.
Mespo expression in the tailbud is clearly excluded from the dorsal-most region. (7) Stage 26. Expression is seen in the tail blastema. Expression in the head
region is a non-specific artifact in this and the next two panels (7–9). (8) Stage 29. (9) Stage 35/36. Mespo is expressed at the tip of the tail. |
