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FIG. 3. pMesogenin1 is zygotically expressed in presumptive mesoderm of gastrulae and tailbud region in Xenopus embryos. (A)RT-PCR analysis of Mesogenin1 expression shows that pMesogenin1 is zygotically expressed and developmentally regulated during
Xenopus embryogenesis. T-box transcription factor VegT, on the other hand, is expressed both maternally and zygotically (Zhang and King, 1996). X-max2 gene expression (King et al., 1993) was also monitored as a control for RT-PCR. (B) Whole-mount in situ analysis of pMesogenin1 expression during Xenopus embryogenesis. During gastrulation, pMesogenin1 expression was detected in ventrolateral mesoderm. After gastrulation, pMesogenin1 is expressed in the tailbud (presomitic mesoderm) region until early tadpole stage. Abbreviations: D, dorsal; V, ventral; A, anterior; and P, posterior. |
