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Fig. 7. PI3-K/AKT signaling regulates Mespo expression. (A) Hatched lines indicate the three caudal regions (C, R, and S) used for western blot of Akt and phosphorylated Akt. (B) Western blot of Akt and phosphorylated Akt in different regions indicated in panel A. (C) Western blot of phosphorylated Akt in the caudal PSM equivalent to region C (illustrated in panel A) treated with either LY294002 or DMSO. (D–G) The neurula stage embryos were treated with either DMSO (D, F), or LY294002 (E, G) for 2 h, and then stained for Mespo and Paraxis as indicated. (H) RT–PCR of Mespo mRNA in the caudal PSM equivalent to region C (illustrated in panel A) treated with either DMSO or LY294002. The caudal PSM were dissected at stage 23 and treated with drugs for 2 h, then harvested for RT–PCR. |
