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Fig. 1. Whole-mount in situ hybridisations of Xenopus embryos showing specific expression patterns of the MMPs-7, -9 and -18 in migrating macrophages (stage 24). Representative examples of the 10 embryos assayed. (A) MMP-7, (B) MMP-9 and (C) MMP-18. (D–F) Double whole-mount in situ hybridisation with POX2 (Di, Ei, Fi) developed with fast red and MMP-7 (Dii), MMP-9 (Eii) and MMP-18 (Fii) developed with NBT/BCIP. Arrows indicate single macrophages stained for both POX2 and an MMP. All embryos are shown in lateral view, with anterior to the left. |
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mmp1 (matrix metallopeptidase 1) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 24, lateral view, anterior left. |