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mid1xenopus   

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Experiment details for mid1

Hedgehog-dependent E3-ligase Midline1 regulates ubiquitin-mediated proteasomal degradation of Pax6 during visual system deve...

Hedgehog-dependent E3-ligase Midline1 regulates ubiquitin-mediated proteasomal degradation of Pax6 during visual system development.

Gene Clone Species Stages Anatomy
mid1.L laevis NF stage 24 midbrain , cement gland primordium , somite , otic placode , optic vesicle , [+]
mid1.L laevis NF stage 32 otic vesicle , midbrain , hindbrain , cement gland , somite , [+]
mid1.L laevis NF stage 32 otic vesicle , midbrain-hindbrain boundary , cement gland , eye , optic vesicle , [+]
mid1.L laevis NF stage 33 and 34 midbrain , hindbrain , cement gland , somite , heart , [+]
mid1.L laevis NF stage 37 and 38 otic vesicle , retina , midbrain , hindbrain , heart , [+]

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  Fig. 1. Mid1 is expressed in cells of the forming optic stalk and induced on shh RNA injection. (A) mid1 mRNA expression was assessed by Wmish on Xenopus laevis embryos at different NF stages. (a–d) Lateral view, (a′) cranial view, and (b′–d′) lateral view. Twenty-micrometer gelatin/albumin sections at the level of the tadpole’s head (d1–d3), heart (c1), neural tube (c2), and pronephric anlage (c3). cg, cement gland; ec, endocardium; ed, eye disk; ev, eye vesicle; hd, hindbrain; he, heart; hf, heart field; kt, kidney tubules; mb, midbrain; mc, myocardium; op, olfactory placode; os, optic stalk; ov, otic vesicle; pnt, pronephric tubule; pos, presumptive os; sm, somites. (c4–c7) mid1 expression in eye stalk region in serial sections. (B) Injections were done into one cell of two-cell stage embryos. (a–d′) Synthetic shh-RNA (500 pg) was injected and Wmish against mid1, pax2, pax6, and ptc1 was performed as indicated. (e–f′) pax2-mo (2.5 pmol) was injected, and expression of mid1 and pax6 was monitored. Shown are transversal sections of the eye at the level of the lens. (g–g′) pax6-mo (2.5 pmol) was injected, and pax2 was monitored. Shown are lateral views of the head region and a transversal section of the head region at the level of the optic vesicles (g′). (h–h′) pax2 RNA (100 pg) was injected and expression of pax6 was analyzed.