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mid1xenopus   

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Experiment details for mid1

MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization.

MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization.

Gene Clone Species Stages Anatomy
mid1.L xl082d10 laevis NF stage 13 ectoderm , epidermis , blastopore
mid1.L xl082d10 laevis NF stage 15 neuroectoderm , blastopore , neural plate , presumptive paraxial mesoderm
mid1.L xl082d10 laevis NF stage 20 cement gland primordium , somite , paraxial mesoderm , anterior neural tube , trunk somite , [+]
mid1.L xl082d10 laevis NF stage 25 retina , brain , midbrain , epidermis , cement gland primordium , [+]
mid1.L xl082d10 laevis NF stage 29 and 30 retina , brain , hindbrain , midbrain-hindbrain boundary , spinal cord , [+]

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  Fig. 1. Expression patterns of xMID1 and xMID2 through early embryogenesis. (A,B) RT-PCR analysis. (A) xMID1 and xMID2 mRNAs are expressed maternally and zygotically. Ornithine decarboxylase (ODC) was used as an internal control. The number above each lane is the embryonic stage. â RT, control experiment without reverse transcriptase. (B) Ectodermal expression of xMIDs in the early neurula (stage 14) Xenopus embryo. In the neural plate, xMID1 and xMID2 were expressed at similar levels to in ventral epidermis (Epidermis) and whole embryo (W.E.). NCAM and Sox2 are pan-neural markers. Epidermal keratin I (Epi. keratin) is an epidermal marker. (C-I) Expression pattern of xMID1 in embryos at the stages indicated (lower right in each panel). (C) Dorso-posterior view, dorsal to the top. (D,E) Dorsal view, anterior to the top. (F) Anterior view, dorsal to the top. (G) Dorsal view, labeled with sense probe (S), anterior to the top. (H,I) Lateral view, anterior to the left, dorsal to the top. (J,K) xMID2 expression. Lateral view, anterior to the left, dorsal to the top. (K) Sense probe (S). cg, cement gland; hb, hindbrain; ht, heart tube; mb, midbrain; nt, neural tube; op, optic vesicle; ot, otic vesicle; pg, pineal gland; pn, pronephros; pp, pharyngeal pouch; pr, proctodeum; so, somite.