|
Gene |
Clone |
Species |
Stages |
Anatomy |
mid1.L
|
xl082d10
|
laevis
|
NF stage 13
|
ectoderm
,
epidermis
,
blastopore
|
mid1.L
|
xl082d10
|
laevis
|
NF stage 15
|
neuroectoderm
,
blastopore
,
neural plate
,
presumptive paraxial mesoderm
|
mid1.L
|
xl082d10
|
laevis
|
NF stage 20
|
cement gland primordium
,
somite
,
paraxial mesoderm
,
anterior neural tube
,
trunk somite
,
[+]
|
mid1.L
|
xl082d10
|
laevis
|
NF stage 25
|
retina
,
brain
,
midbrain
,
epidermis
,
cement gland primordium
,
[+]
|
mid1.L
|
xl082d10
|
laevis
|
NF stage 29 and 30
|
retina
,
brain
,
hindbrain
,
midbrain-hindbrain boundary
,
spinal cord
,
[+]
epidermis
,
cement gland
,
presomitic mesoderm
,
pronephric duct
,
eye
,
optic vesicle
,
ventral
,
heart primordium
,
pronephric kidney
,
tail somite
,
tail tip
|
Display additional annotations [+]
|
|
Fig. 1. Expression patterns of xMID1 and xMID2 through early embryogenesis. (A,B) RT-PCR analysis. (A) xMID1 and xMID2 mRNAs are expressed maternally and zygotically. Ornithine decarboxylase (ODC) was used as an internal control. The number above each lane is the embryonic stage. â RT, control experiment without reverse transcriptase. (B) Ectodermal expression of xMIDs in the early neurula (stage 14) Xenopus embryo. In the neural plate, xMID1 and xMID2 were expressed at similar levels to in ventral epidermis (Epidermis) and whole embryo (W.E.). NCAM and Sox2 are pan-neural markers. Epidermal keratin I (Epi. keratin) is an epidermal marker. (C-I) Expression pattern of xMID1 in embryos at the stages indicated (lower right in each panel). (C) Dorso-posterior view, dorsal to the top. (D,E) Dorsal view, anterior to the top. (F) Anterior view, dorsal to the top. (G) Dorsal view, labeled with sense probe (S), anterior to the top. (H,I) Lateral view, anterior to the left, dorsal to the top. (J,K) xMID2 expression. Lateral view, anterior to the left, dorsal to the top. (K) Sense probe (S). cg, cement gland; hb, hindbrain; ht, heart tube; mb, midbrain; nt, neural tube; op, optic vesicle; ot, otic vesicle; pg, pineal gland; pn, pronephros; pp, pharyngeal pouch; pr, proctodeum; so, somite. |