|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
pax6
|
|
laevis
|
NF stage 31
|
retina
,
forebrain
,
diencephalon
,
midbrain
,
hindbrain
,
[+]
|
snai2
|
|
laevis
|
NF stage 35 and 36
|
neural crest
,
melanophore
,
neural tube
|
rax
|
|
laevis
|
NF stage 31
|
otic vesicle
,
retina
,
pineal gland
|
en1
|
|
laevis
|
NF stage 28
|
midbrain-hindbrain boundary
,
neuroectoderm
,
neural tube
|
lbx1
|
|
laevis
|
NF stage 28
|
hindbrain
,
spinal cord
,
mesoderm
,
myotome
,
neural tube
,
[+]
|
|
|
Figure 5. Rbmx is required for normal eye development and neuronal differentiation. A-H: Embryos injected unilaterally with Rbmx MO and cultured to stage 31 (A-D), stage 28 (E-F), or stage 35 (H). A,B: In situ hybridization for Xrx1. C,D: In situ hybridization for Pax6; red lines indicate Pax6 expression domain in normal forebrain (C), which is absent in the Rbmx MO injected side (D). E-H: Dorsal view of head and anterior trunk of Rbmx morphants. Injected side is to the right. E-H: In situ hybridization using antisense probe for En1 (E), Lbx1 (F), Lim1 (G), and Slug (H). Red arrowhead in F indicates Lbx1 expression in hypaxial muscle on the uninjected side. Yellow line in H indicates the midline and red arrow indicates melanocytes on the uninjected side. Notably Slug expression is absent in the neural tube and trunk on the injected side. |