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Fig. 7. EnR-LefδN-GR755A generates reduced expression of pronephric mesenchyme markers. EnR-LefδN-GR755A (0.5 ng) with rhodamine dextran tracer was injected into single V2 blastomeres at the eight-cell stage and dexamethasone was added at stage 16. Embryos were fixed at stage 23 (A and B), stage 25 (C and D), and stage 27 (E and F). The injected side of embryos shows reduced expression of pronephric mesenchyme markers XPax8 (pax8) (A), XLim1 (lhx1) (C), and vHNF1 (hnf1β) (E), shown via whole-mount colorimetric or fluorescent in situ. The control side of embryos shows expected normal distribution of pronephric mesenchyme markers (B, D, F). Injected and control side images shown are from the same embryo and insets are enlargements of the pronephric region. |
