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Figure 6
Whole-mount staining of the pronephros anlage by Xlim-1. (a and b) Whole-mount in situ hybridizations using an Xlim-1 antisense probe on HNF1β- and HNF1α-injected embryos. The left and right side of the same animal is shown with the cement gland (cg) marked to indicate the anterior part of the stage 20 tail-bud embryos. The arrow points to the pronephros anlage stained by Xlim-1 hybridization. The staining involved 8, 9, or 4 embryos injected with HNF1α, HNF1β, or P329L329 (not shown) and was indistinguishable from 10 uninjected embryos. |
