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Fig. 7. SU5402 treatment blocks pronephric primordium development by stage 22 but does not block glomeral development. (A, B) Control and SU5402 treated (from stage 15 to 22) embryos stained with lim1. SU5402 treatment has completely blocked lim1 expression in the pronephros. (C–F) Nephrin expression in controls and embryos treated with 50 μM SU5402 from stage 15 to 32. The glomus is abnormally shaped but is still present, as it is in embryos injected with hh. A second, previously unrecorded site of nephrin expression was observed in the branchial arches. This expression is also retained in SU5402-treated embryos but is in an abnormal position indicating an alteration in neural crest migration (red arrows). Panels E and F are enlarged views of the same embryos as shown in panels C and D. |
