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lhx1xenopus   

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Experiment details for lhx1

Beck CW and Slack JM (1998) Assay

Analysis of the developing Xenopus tail bud reveals separate phases of gene expression during determination and outgrowth.

Gene Clone Species Stages Anatomy
lhx1.L laevis NF stage 14 intermediate mesoderm , upper blastopore lip , axial mesoderm , neural groove

  lhx1 (LIM homeobox 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 14, dorsal view, anterior left.

Gene Clone Species Stages Anatomy
lhx1.L laevis NF stage 15 to NF stage 29 and 30 brain , forebrain , midbrain , hindbrain , spinal cord , [+]

  lhx1 (LIM homeobox 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 29 & 30, lateral view, anterior left, dorsal up.

Gene Clone Species Stages Anatomy
lhx1.L laevis NF stage 29 and 30 spinal cord , notochord , chordoneural hinge , tail tip

  lim1 gene expression in Xenopus laevis embryo, tail region only, assayed via in situ hybridization, NF stage 29 & 30, lateral view, anterior left, dorsal up.

Gene Clone Species Stages Anatomy
lhx1.L laevis NF stage 32 notochord , tail bud , chordoneural hinge

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  Fig. 6. Expression of early genes in the chordoneural hinge. (A) Sagittal section of a stage 32 tail bud whole mount in situ hybridisation to show expression of Xnot2 (dark staining). Transcripts are restricted to the chordoneural hinge and posterior ventral neural tube as previously reported (Gont et al., 1993). (B) schematic diagram of Xnot2 expression based on (A). (C) Sagittal section of stage 30 tail bud to show Xlim1 expression in the chordoneural hinge and posterior notochord. There is also punctate expression of Xlim1 in the neural tube more anteriorly. Xlim1 transcripts are clearly absent from the tip of the chordoneural hinge in the sagittal section, suggesting that expression is excluded from the M region. (D) Schematic diagram of Xlim1 expression in the tail bud based on (C). (E) Sagittal section of stage 30 tail bud to show Xshh expression in the chordoneural hinge, notochord and floorplate of the neural tube and underlying endoderm. (F) Schematic diagram of Xshh expression based on (E). Scale bar in (A,C,E), 250 mm. Abbreviations: cnh, chordoneural hinge; end, endoderm; nc, notochord; nt, neural tube; pw, posterior wall.