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Figure 3. Inhibiting non-canonical wnt signals or promoting canonical wnt signals leads to increases in cell division, while promoting non-canonical or suppressing canonical signals results in a decrease in division rates. Controls are shown on the left, and treated embryos on the right. The pronephros is stained green by means of fluorescent in situ hybridization (FISH), dividing cells stained red by means of anti-phospho-H3, and mRNA-injected samples identified by blue green fluorescent protein (GFP) staining in all panels. Three-dimensional scanning determined which phospho-H3 positive cells were within pronephroi in each case (Table 2). A–F: Images show contralateral sides of the same embryos. G,H: Representative images with average division rates were selected as both sides of each embryo received the same treatment. Scale bars = 100 microns. |
