| |
Fig. 8. rfng overexpression caused ectopic proximal pronephrogenesis. (A-I) X. laevis embryos were injected with rfng mRNA as previously described. rfng overexpression causes ectopic Lim-1 (A) and hrt1 expression (B) at stage 28. At stage 41, 3G8 and 4A6 immunostaining is ectopic; frequently the entire proximal pronephros is duplicated in the distal region (white arrow, C). Similarly, Na+K+ATPase (D), slc5a2 (E) and nephrin (F) expression is also ectopic. GATA-3, which is expressed solely in the distal tubule, is completely absent (G). Co-injection of rfng mRNA with the wnt4 MO abrogates these effects; nephrin (H) and slc5a2 (I) expression is reduced in these embryos. Asterisk denotes injected side. |
