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Fig. 6. xSyndecan1 regulates dorso-ventral patterning of the ectoderm. (A–F) xSyn1 knockdown increased neural plate wide and reduced non-neural ectoderm. Embryos at the two-cell stage were microinjected in both blastomeres with (A, D) control and (B, E) xSyn1 morpholinos (34 ng), raised until neurula stage and in situ hybridization against (A, B) sox2 and (D, E) cytokeratin performed. Neural plate width was measured at the half of the antero-posterior axes with ImageJ. Statistical analysis with Kruskal–Wallis test and Dunn's post test showing that means vary significantly with (C) p < 0.01 and (F) p < 0.05, respectively. The number of embryos used for each experimental point is indicated above each bar. (G, H) xSyn1 is necessary for epidermal integrity. Embryos at the two-cell stage were microinjected in both blastomeres with control and xSyn1 morpholinos (34 ng) and raised until tailbud stage. Arrows point where epidermis is affected in xSyn1 morphant embryos. |
