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Fig. 4. Perturbation of XOct-25 function in the ectoderm during embryogenesis. The phenotypes of neurula stage embryos coinjected with either Oct MO or 6-mismatch control MO together with β-gal mRNA into one of the dorsal animal blastomeres at the 8-cell stage. The amounts of injected antisense oligonucleotides per embryo were 5.6 ng (A–C) and 4.3 ng (G–P) for moderate amounts, and 8.6 ng (D–F) as a large amount. The reduction of Sox2 expression by Oct MO injection was rescued by coinjection of δBMPR mRNA (500 pg, C) or GR-mutXOct-25 mRNA (250 pg, F). Note that cells receiving Oct MO expressed the epidermal markers epidermal keratin and Dlx3 at the expense of the neural marker Sox2 (B, H, I, K and L). There was no effect on the expression of the mesodermal markers chordin and MyoD by XOct-25 knockdown (N and P). The expression of marker genes is shown in purple. β-gal was stained in red and the injected side of the embryo is indicated by brackets. I and L are magnifications of H and K, respectively. The edges of ectopic epidermal marker expression are indicated by dashed lines (I and L). A–F, O and P: dorsal view; G–L and N: frontal view; M: dorso-frontal view. |
