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FIG. 4. Expression of Xepsin (A, C, E, G, I, K) and epidermal keratin (B, D, F, H, J, K) in axis-perturbed embryos analyzed by whole-mount in situ hybridization. All embryos were allowed to develop until control embryos reached stage 23. (A, B) Normal embryos. (C, D) UV-irradiated embryos (DAI 0). (E, F) UV-irradiated embryos (DAI 1). (G, H) LiCl-treated embryos (DAI 10). (A) Blastopore is to the right. (I, J, K) Exogastrula embryos. Ectodermal half is on the top right. (K) Double in situ hybridization showing Xepsin expression (purple) in the more anteriorly confined domain than keratin (pink). |
