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krt12.4xenopus   

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Experiment details for krt12.4

Lim CY et al. (2013) Assay

Optimal histone H3 to linker histone H1 chromatin ratio is vital for mesodermal competence in Xenopus.

Gene Clone Species Stages Anatomy
krt12.4.L laevis NF stage 10 ectoderm , epidermis , animal hemisphere

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  Fig. 2. H3.3/H3-depleted Xenopus embryos fail to express mesodermal marker genes. (A-I′) Control and injected embryos fixed at early gastrula were subjected to RNA in situ hybridization for the analysis of mesodermal (A-C′), endodermal (D-F′) and ectodermal (G-I′) marker gene expression. Representative embryos from three experiments are shown. (A-D,G) Vegetal views; (E,F) lateral views of bisected embryos, dorsal towards the right; (H) lateral view; (I) animal view. (J) Expression of selected genes in control and H3 MO-injected embryos at stage 10.5 was measured by qRT-PCR. All values were normalized to ornithine decarboxylase (ODC) and plotted relative to the respective transcript levels in control embryos. Error bars indicate s.d. of three independent experiments. *P<0.05 using a two-tailed Student t-test.