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Fig. 4. Cement gland patterning. Double label in situ hybridization was carried out on stage 26 control embryos using digoxygenin labeled XCG (in red) and fluorescein-labeled XA (in dark blue) antisense RNA probes (a). Single in situ hybridization was carried out on stage 26 embryos using digoxygenin-labeled XA and XK81 probes (b). (a) Anterior view of a stage 26 embryo showing XCG transcripts in red, XA transcripts in blue. (b) Medial longitudinal sections of the heads of XA-(left) and XK81- (right) stained embryos. Black arrowheads indicate cement gland; white arrowhead indicates hatching gland; small white arrows indicate the epidermis, e, that surrounds the cement gland. Scale bar in a, 200 mm; embryos in b are at twice that magnification. |
