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Fig. 3. Gene expression in the Xerl-injected embryo. Synthesized mRNAs of GFP or Xerl were injected into one dorsal blastomere of 4-cell stage embryos, and neural marker gene expression was examined by whole mount in situ hybridization at the stage 15 (A- E) or 20 (F and G). (A) XlSox-2 expression showing the neural plate area (dorsal view). Arrowhead indicates expansion of the XlSox-2-positive area in the Xerl-injected side. (B) XK81A1 expression showing the epidermal area (dorsal view). Arrowheads indicate the posterior end of the head neural plate. (C) NCAM expression showing the neural plate area (anterior view). Arrowhead indicates expansion of NCAM expression. (D) ADAM13 expression showing future cephalic neural crest cells (anterior view). Arrowheads indicate the posterior ends of neural crest cells. (E) Xslug expression showing neural crest cells (dorsal view). Arrowhead indicates the outer and posterior shift of neural crest cells in the Xerl injected side. (F) ADAM13 expression showing cephalic neural crest cells. ADAM13 expression was not seen at the anterior disclosure. (G) XlSox-2 expression (st. 20, anterior view). XlSox-2 expression is seen in the Xerl-injected area (arrow). (H) Cross section at the midbrain level. The dorsal midline is indicated with the broken line. The opened arrowhead shows XlSox-2 expression in the abnormal tissues in the Xerl-injected side. Numbers in each picture indicate the frequency of the corresponding phenotype. |
