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Figure 5. delta-Np63(Ct)-GFP acts as a dominant negative of delta-Np63. A–F: One dorsal blastomere of eight-cell stage embryos was injected with 0.5 ng (F), 2.0 ng (F), or 3.5 ng (A–C,F) of delta-Np63(Ct)-GFP mRNA or with different combinations of delta-Np63(Ct)-GFP and delta-Np63 (D–F). Embryos were fixed at stage 16 and the expression of several genes was analyzed. The injected side is evidenced by green fluorescent protein (GFP) fluorescence. A–C: Dorsal view, anterior at the top. A′–C′: Transverse sections. Dashed lines indicate the midline of embryos. Brackets indicate the width of the neural plate. The injected side of the embryos treated with delta-Np63(Ct)-GFP shows a reduction in XK81a expression (A,A′) and an increase in Sox2 (B,B′) and FoxD3 (C,C′) expression. D,E: The coinjection of delta-Np63(Ct)-GFP and δNp63 (1:1 ratio) rescued XK81a (D) and Sox2 (E) expressions. F: Quantification of delta-Np63(Ct)-GFP dose effects and rescue experiments. np, neural plate.Download figure to PowerPoint |