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Experiment details for krt12.4

Differential distribution of competence for panplacodal and neural crest induction to non-neural and neural ectoderm.

Differential distribution of competence for panplacodal and neural crest induction to non-neural and neural ectoderm.

Gene Clone Species Stages Anatomy
krt12.4.L laevis NF stage 18 epidermis , non-neural ectoderm

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  Fig. 7. Dlx3 promotes different non-neural fates depending on the signaling environment. (A-B′) Keratin (A,A′) and Six1 (B,B′) expression in neural plate stage embryos after unilateral injection (lower half; marked by light-blue β-galactosidase staining) of Dlx3 mRNA and treatment with DMSO (A,B) or the BMP signaling antagonist dorsomorphin (A′,B′). Ectopic Keratin expression in the neural plate is strongly reduced, while ectopic Six1 expression is strongly enhanced by dorsomorphin treatment (inset in B′ shows another example). (C) Effects of signaling agonists or antagonists on ectopic expression of Keratin or Six1 in the neural plate (NP). After Dlx3 mRNA injection, the increase in ectopic Six1 expression by dorsomorphin was blocked by the Wnt agonist azakenpaullone and the FGF antagonist SU5402, whereas co-injection of FGF8 had no significant effect. After GATA2 mRNA injection, dorsomorphin did not increase ectopic Six1 expression, whereas Keratin (not shown) was never ectopically expressed (Fisher’s exact test; *P≤0.05, n.s., not significant). (D-E′′) Transverse sections through neural plate of embryos shown in A (D-D′′) and the inset of B′ (E-E′′). Sections are shown in brightfield (D,E) in the green fluorescent channel, showing mycGFP-positive Dlx3-injected cells (D′,E′); in the red fluorescent channel, showing Sox3 immunostaining (D′,E′); and in overlay (D′′,E′′). DAPI-stained nuclei are shown for orientation in all panels. Ectopic Keratin and Six1 expression is confined to Dlx3-injected cells (asterisks), whereas residual Sox3 staining on injected side is found only in cells that did not receive Dlx3 (arrows). not, notochord.

Gene Clone Species Stages Anatomy
krt12.4.L laevis NF stage 18 epidermis , non-neural ectoderm

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  Fig. S3. Effects of Dlx3 or GATA2 loss of function after injection of engrailed repressor constructs on neural and non-neural ectodermal markers. Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. See text for details.

Gene Clone Species Stages Anatomy
krt12.4.L laevis NF stage 22 epidermis , non-neural ectoderm

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  Fig. 5. Effects of Dlx3 or GATA2 gain or loss of function on non-neural ectodermal markers. (A-G′′) Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows) and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural (orange) ectoderm compared with the control side (arrowheads) are indicated. Insets depict additional embryos with ectopic expression of Six1 (A), Eya1 (B) and Dlx5 (D) in central neural plate.