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Figure 4. BMP4 can induce intermediate neural plate tissue to form RB sensory neurons
In situ hybridization of intermediate neural plate explants (A-D,F) or epidermal ectoderm (E) isolated at stage 11.5−12 and subsequently incubated with a heparin bead with or without hBMP4 (A-D,F) until stage-matched controls reached stage 24−25. (A) Intermediate explants incubated with a 0.5MBS-soaked bead never expressed the RB marker XHox11L2. Intermediate explants incubated with 1 ng/ml BMP4 stained positive for XHox11L2 (arrows in B) and XN-tubulin (arrows in C; white circle indicates position of the bead observed as an impression, since the bead was displaced during processing). (D) Expression of XSox2 in explants incubated with 1ng/ml BMP4 beads suggests that neural tissue is maintained in the area around the beads. (E) Epidermal explants stained positive for XKeratin, while neural plate explants incubated with 1 ng/ml BMP4 did not (F). |
