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Fig. 6. Crucial time window of Xfoxi1a. GR-Xfoxi1a mRNA (50 pg/cell) was injected into two unilateral animal blastomeres of eight-cell embryos. Dex was added at stage 11 (B,E,H,K) or stage 13 (C,F,I,L). Embryos were harvested at stage 15 and used for whole-mount in situ hybridization with a probe for Sox2 (A-C), XK81 (D-F), FoxD3 (G-I) or Six1 (J-L). Embryos without Dex treatment (A,D,G,J) were used as the negative control. Arrowheads indicate the injected side. (M) Flag-tagged GR-Xfoxi1a mRNA (50 pg/cell) was injected into all the animal blastomeres of eight-cell embryos. Animal caps were excised at stage 9 and cultured until stage 11, 13 or 15. The intact form of flag-GR-Xfoxi1a was detected by western blot analysis. Hsp70 was used as the loading control. |
