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Figure 2. neptune is expressed in the hatching gland, cement gland, and prospective VBI. (a) A stage-11 embryo, dorsal is to the right. neptune is expressed in the ectoderm of the animal pole but is being excluded from the future neural plate. Arrowheads show the location of the blastopore lip on the dorsal and ventral sides. (b) At stage 12, neptune is expressed in the cement gland primordium. At (c) stage 18 and (d) stage 22, there is expression in the cement and hatching glands. (b)–(d) are anterior views of the embryos. (e) A dorsal view of a stage-15 embryo shows ectodermal expression along the edge of the neural folds. (f,g) Tailbud stages. (h) and (i) show expression in the VII and IX cranial ganglia in stage-25 and stage-31 embryos, respectively. Ventral views of (j) stage-22 and (k) stage-25 embryos show expression in the VBI and around the remnant of the blastopore. neptune expression is excluded from the liver anlage (LA). (l) A lateral view of a stage-31 embryo. (m) Stage-37/38 embryo with circulating cells expressing neptune |
