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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
pmp22
|
|
laevis
|
NF stage 15
|
cement gland primordium
,
paraxial mesoderm
|
pmp22
|
|
laevis
|
NF stage 26
|
presomitic mesoderm
,
somite
,
trigeminal ganglion
,
profundal placode
,
caudal presomitic mesoderm
|
pmp22
|
|
laevis
|
NF stage 28
|
presomitic mesoderm
,
somite
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
[+]
|
pmp22
|
|
laevis
|
NF stage 35 and 36
|
somite
,
pharyngeal arch
,
head
,
tail somite
|
sox9
|
|
laevis
|
NF stage 26
to
NF stage 32
|
otic vesicle
,
brain
,
mandibular crest
,
hyoid crest
,
branchial crest
,
[+]
|
sox9
|
|
laevis
|
NF stage 35 and 36
|
otic vesicle
,
brain
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
[+]
|
|
|
Fig. 2. Expression of Pmp22 in whole-mount in situ hybridization. (A) Expression of Pmp22 at stage 15 in the cement gland. Anterior view, dorsal to top. (B) At stage 22,
Pmp22 was detected in the developing somites and profundal and trigeminal nerves. (C) At stage 22 Pmp22 sense probe was used as a negative control. (D) Pmp22 was
expressed in the profundal and trigeminal ganglia, cranial mesenchyme, and somites at stage 29/30, where it was also beginning to be expressed in the branchial arches.
(E) Strong expression of Pmp22 was observed in the craniofacial region. (F–G, I) To confirm the tissue-specific expression of Pmp22, we also performed in situ hybridization
for Sox9 and Islet1. (H, I) At stage 35, both Pmp22 and Sox9 were detected in the branchial arches. Panels (B–I), lateral view, anterior to the left. ba, branchial arches; cg,
cement gland; pr, profundal ganglia; s, somites; tg, trigeminal ganglia. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
otx2
|
|
laevis
|
NF stage 40
|
otic vesicle
,
ventral
|
pmp22
|
|
laevis
|
NF stage 15
|
|
pmp22
|
|
laevis
|
NF stage 35 and 36
|
roof plate
,
eye
,
posterior neural tube
,
floor plate
,
anterior neural tube
,
[+]
|
pmp22
|
|
laevis
|
NF stage 40
|
otic vesicle
,
lens
,
brain
,
trachea
,
eye
,
[+]
|
wnt3a
|
|
laevis
|
NF stage 40
|
otic vesicle
,
dorsal
|
|
|
Fig. 3. Expression of Pmp22 determined by in situ hybridization of whole sections. (A) At stage 15, Pmp22 was weakly expressed in mesoderm cells. (B) At stage 30 Pmp22
sense probe was used as a negative control. (C–D) Transverse sections showing the expression of Pmp22 in the mesenchyme of the head at stage 35. Expression of Pmp22
was detected in the eye, craniofacial cartilage, trigeminal ganglia, and roof plate of the anterior neural tube, as well as floor plate of the posterior neural tube. (E) Schematic
representation of the head of a stage 40 embryo as viewed from the lateral side, dorsal to top, and anterior to the left. Image was modified from Nieuwkoop and Faber
(1967). (F–L) At stage 40, transverse sections revealed expression of Pmp22 in the head mesenchyme, floor plate of the developing brain, cartilage (F–I), lens (G), trigeminal
ganglia (H), otic vesicle (I, L), developing trachea (J) and lungs (K). (L–O) At stage 40, expression of Pmp22 was observed in the dorsal (blue arrow) and ventral (green arrow)
aspects of the developing otocyst. Islet1 (bracket) is expressed in the sensory organs of the otic vesicle (O), which did not overlap with Pmp22 (L). bc, basihyal cartilage; br,
branchial cartilage; cc, craniofacial cartilage; ce, cerathoyal cartilage; di, diencephalon; et, ethmoid-trabecular cartilage; fp, floor plate; he, heart; le, lens; lu, lung; me, Meckel’s
cartilage; no, notochord; ov, otic vesicle; ph, pharynx; qu, quadrate cartilage; rp, roof plate; st, stomodeum; tg, trigeminal ganglion; tr, trachea. The scale bar represents
100 μm. |