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isl1xenopus   

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Experiment details for isl1

Neural crest migration requires the activity of the extracellular sulphatases XtSulf1 and XtSulf2.

Neural crest migration requires the activity of the extracellular sulphatases XtSulf1 and XtSulf2.

Gene Clone Species Stages Anatomy
isl1 tropicalis NF stage 28 heart , pineal gland , trigeminal placode , cranial placode , profundus ganglion

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  Fig. 3. Overexpression of XtSulf2 affects the expression of a number of genes expressed in anterior neural tissue and the cranial neural crest. One blastomere of a two-cell Xenopus tropicalis embryo was injected with 0.5 ng of XtSulf2 mRNA, embryos were allowed to develop to NF stage 28 and fixed for in situ hybridization using En2 (A–B), Isl1 (C–D), Krox20 (E–G), Lim1 (H–I), NeuroD (J–L), Otx2 (M–N), Pax6 (O–Q), Slug (R–S), Sox3 (T–U), and Sox8 (V–W). En2 expression is shifted posteriorly on the injected side (A–B). Isl1 expression in the profundal and trigeminal placodes shifts posteriorly (black arrowheads) as does the expression in the heart (C–D). Krox20 expression in the migrating neural crest shifts posteriorly (E–F white arrowheads) as does the expression in r5 (G black lines). Lim1 expression in the pronephros is restricted anteriorly (H–I black lines). NeuroD expression in the profundal and trigeminal placodes is disrupted (J–K red arrowheads), and expression in the olfactory placodes fuses with expression in the eye placodes (L yellow arrowheads). Otx2 expression is restricted in the midbrain (M–N black lines). Pax6 expression is ectopically expressed in the posterior and dorsal head ectoderm (O–P–Q red asterisk). Slug expression at the leading edge of neural crest migration remains closer to the neural tube than in controls (R–S black lines). Sox3 expression is decreased in neural crest but expands laterally (T–U black lines). Sox8 expression in the anterior and posterior branchial arches is decreased and the leading edge of the migrating neural crest remains closer to the neural tube than in controls (V–W green arrowhead). (A–B, G, Q) are dorsal views of embryos, anterior at the top. (C–F, H–K, M–P, R–W) are lateral views of embryos, anterior to the left. (L) is an anterior view of embryo in (J–K), dorsal at the top. Asterisks indicate injected side. CS control side, IS injected side.