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Experiment details for ins

Yuan L et al. (2014) Assay

IRE1α is essential for Xenopus pancreas development.

Gene Clone Species Stages Anatomy
ins.L laevis NF stage 43 pancreas , dorsal pancreatic bud

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  Fig. 4. IRE1α knockdown specifically inhibits the expression of differentiation marker genes. Whole mount in situ hybridization analyses revealed that the expression of insulin (B) and amylase (D) was not detected in IRE1α MO injected embryos at stage 43 compared with control MO injected embryos (A, C). The white arrows point to the positive staining of insulin and amylase.

Gene Clone Species Stages Anatomy
ins.L laevis NF stage 43 pancreas

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  Fig. 7. IRE1α knockdown inhibits pancreas marker gene expression.Whole mount in situ hybridization analyses revealed that the expression of insulin (B) and amylase (D) was not detected in 50 ng XBP1MO injected embryos at stage 43 compared with control MO injected embryos (A,C). The white arrows indicate positive staining.