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Fig. 1. Xenopus as model system to study monogenic diabetes mutations. (A) Examples illustrating different type of assays that are used in Xenopus embryos to studypancreatic phenotypes. Abbreviations: AE, anterior endoderm; D, dorsal; DN, dominant negative; DP, dorsal pancreas; ISH, in situ hybridization; IF, immunofluorescencestaining; MO, morpholino; PE, posterior endoderm; V, ventral, VP, ventral pancreas. (B) Schematic representation of different type of assays performed in Xenopus oocytesto study monogenic diabetes mutations, including SCL2A2 [133]. WFS1 [134], ABCC8 [135] and KCNJ11 [100,101,125–127] mutations. |