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FIG. 2. Colocalization of XlHbox 8 and islet hormones in adult frog pancreas. Sections of frog pancreas were immunostained with XlHbox
8 antibody, detected using alkaline phosphatase·linked secondary antibody {A, C, E), and then immunostained with hormone antibodies
detected with fluorescently labeled secondary antibody (B, D, F). Arrowheads indicate representative cells coexpressing XlHbox 8 and
hormone. (A, Bl XlHbox 8 :md insulin colocalization in an islet cell cluster. (C, Dl XlHbox 8 and glucagon colocalization near the pancreatic
duct. IE, F) XlHbox 8 and somatostatin co\ocalization in an endocrine cell cluster. 500 cells positive for each hormone were scored for
nuclear XlHbox 8. Bar, 50 .urn. |