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Fig. 6. Histological analysis of molecular markers in stage 446 Xenopus
tadpoles. A) Stained transverse section of a stage 44 tadpole.
B) Stained sagittal section of a stage 44 tadpole. C) Sagittal section of a
stage 44 tadpole, similar to the bracketed area in E, stained with an
anti-insulin antibody. D) Stained sagittal section of a stage 44 tadpole,
more medial than the section in B. E) Sagittal section of a stage 44
tadpole, similar to the bracketed area in D, stained with an anti-carboxypeptidase
A (carbA) antibody. F) Stained transverse section of a
stage 46 tadpole. G) Transverse section of a stage 46 tadpole, similar to
the bracketed area in F, stained with an anti-somatostatin (som) antibody.
Arrows point to positively stained cells in the duodenum. H) Transverse
section of a stage 46 tadpole that is slightly more posterior to the section
in F, allowing one to see the distal end of the stomach joining with the
duodenum. The section is stained with an anti-carboxypeptidase A antibody.
I) Pax6 RNA expression in a sagittal section of a stage 45 tadpole.
J) NeuroD RNA expression in a sagittal section of a stage 45 tadpole.
Approximate planes of section are shown in the schematic diagram.
Abbreviations: d, duodenum; gb, gall bladder; int, intestine; lv, liver; lu,
lung; nc, notochord; nt, neural tube; oe, oesophagus; p, pancreas (blue);
pn, pronephros; s, somite; st, stomach. |
