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Fig. 5. Histological analysis of pancreas development in stage 41 and
stage 42 Xenopus tadpoles. A) Stained transverse section of a stage 41
tadpole showing the ventral pancreas. B) Transverse section of a stage
41 tadpole, similar to the one in A, stained with an anti-insulin antibody.
Note the absence of insulin expression in the ventral pancreas. C) Stained
transverse section of a stage 41 tadpole, more posterior than the section
in A, showing the dorsal pancreas. D) Transverse (slightly oblique) section
of a stage 41 tadpole, similar to the one in C, stained with an anti-insulin
antibody. E) Stained sagittal section of a stage 41 tadpole showing where
the dorsal and ventral pancreas fuse. F) Close-up of the bracketed area
in E. G) Stained sagittal section of a stage 42 tadpole. H) Sagittal section
of a stage 42 tadpole, similar to the bracketed area in G, stained with an
anti-insulin antibody. Note how insulin expression is localized to one
region of the pancreas, which presumably is the portion derived from the
dorsal pancreas. Approximate planes of section are shown in the schematic
diagrams. Abbreviations: a, aorta; d, duodenum, dp, dorsal pancreas
(dark blue); h, heart; int, intestine; lv, liver; nc, notochord; nt, neural
tube; oe, oesophagus; ph, pharynx; pn, pronephros; s, somite; st, stomach;
vp, ventral pancreatic rudiments (light blue). |