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Fig. 4. Insulin mRNA in situ hybridization of control and transgenic pancreases at varying stages. These reactions were carried out together and developed for the same time. (A) Control premetamorphic pancreas has small islets that stain for insulin mRNA with similar intensity as (D) adult islet cells. (B) At NF62 most of the insulin mRNA is down regulated and reexpression begins at (C) the end of climax NF66. The pInsulin-TRDN transgenic pancreas does not down regulate insulin mRNA at (E) NF62 or (F) NF66, but its ability to aggregate by NF66 is not impaired. (G) pElastase-TRDN-GFP at NF66 showed similar insulin mRNA down regulation as that of wild-type but the β-cells do not aggregate. Scale bar equals 40 μm. |