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Fig. 8. Overexpression of tm4sf3 mRNA resulted in annular pancreas development. (A,B) Expression of the acinar cell maker elastase is detected in the ectopic pancreatic tissue (n=19). (D,E) The endocrine marker insulin is not expressed (n=24). (G,H) Development of the liver was not affected by tm4sf3 overexpression (n=17). The annular pancreas is outlined. (J,K) Schematic illustrating the development of annular pancreas. Pink, liver; blue, pancreas; yellow, GI tract. The pancreas normally grows behind the stomach, but in tm4sf3-injected embryos the pancreas also grows around the front of the duodenum. Lighter shading behind the yellow indicates the pancreas. (C,F,I) Representative serial sections from tm4sf3-injected whole gut. The pancreas encircles the stomach completely (blue outline in F). The stomach also appears slightly affected. (L) Three-dimensional reconstruction of histological sections illustrates the development of annular pancreas. |
