| |
Fig. 6. Whole-mount immunohistochemical analysis of hsp30 protein accumulation in Xenopus embryos. Late tailbud (stage 35; A,B) and early tadpole (stage 41/42) embryos were incubated at either 22°C (A,C)
or 33°C (B,D) for 2 h. The embryos were then processed for
immunohistochemical analysis using an affinity-purified polyclonal
hsp30 antibody as outlined in Materials and Methods.
The outer portion of the tailfin was quite transparent in
the late tailbud stage embryos and is not readily visualized in
the photograph in C. CG, cement gland; LP, lens placode;
SR, somitic region; P, proctodeum. |
