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hsp30cxenopus   

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Experiment details for hsp30c

Spatial pattern of constitutive and heat shock-induced expression of the small heat shock protein gene family, Hsp30, in Xen...

Spatial pattern of constitutive and heat shock-induced expression of the small heat shock protein gene family, Hsp30, in Xenopus laevis tailbud embryos.

Gene Clone Species Stages Anatomy
hsp30c.L laevis NF stage 28 ectoderm , cement gland , cement gland primordium , somite , lens placode , [+]

  Fig. 3. Histological analysis of hsp30 mRNA accumulation in sections from Xenopus tailbud embryos. Whole-mount in situ hybridization with DIG-labeled hsp30 antisense riboprobe was performed on control and heat shocked (1 h at 33°C) mid- (stage 28) and late (stage 35) tailbud embryos followed by histological analysis as described in Materials and Methods. A,B: Anterior sections of control and heat shocked midtailbud embryos, respectively, showing the cement gland. C: Cross-section displaying the proctodeum in heat shocked midtailbud embryos. D: An enlarged medial region of a heat shocked midtailbud whole-mount embryo (stage 28) hybridized with DIG-labeled hsp30 antisense riboprobe and demonstrates the abundance of hsp30 mRNAin the somites. E,F: The presence of hsp30 mRNAin the somitic region of heat shocked mid- and late tailbud embryos, respectively. G,H: Cross-sections of the eye of control and heat shocked midtailbud embryos. CG, cement gland; M, mesencephalon; LP, lens placode; OC, optic cup; N, notochord; S, somites; HG, hindgut; P, proctodeum; E, ectoderm.