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Fig. 3. Histological analysis of hsp30 mRNA accumulation in
sections from Xenopus tailbud embryos. Whole-mount in situ hybridization
with DIG-labeled hsp30 antisense riboprobe was performed on
control and heat shocked (1 h at 33°C) mid- (stage 28) and late (stage
35) tailbud embryos followed by histological analysis as described in
Materials and Methods. A,B: Anterior sections of control and heat
shocked midtailbud embryos, respectively, showing the cement gland.
C: Cross-section displaying the proctodeum in heat shocked midtailbud
embryos. D: An enlarged medial region of a heat shocked midtailbud
whole-mount embryo (stage 28) hybridized with DIG-labeled
hsp30 antisense riboprobe and demonstrates the abundance of hsp30
mRNAin the somites. E,F: The presence of hsp30 mRNAin the somitic
region of heat shocked mid- and late tailbud embryos, respectively.
G,H: Cross-sections of the eye of control and heat shocked midtailbud
embryos. CG, cement gland; M, mesencephalon; LP, lens placode; OC,
optic cup; N, notochord; S, somites; HG, hindgut; P, proctodeum; E,
ectoderm. |