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Figure 1. A: Preparation of Xenopus recombinant limbs. Recombinants with whole limb bud mesenchyme at stage 55–56 formed many digits. B–G: Gene expressions in recombinants. Expressions of fgf-8 (B), shh (C), and Hoxa-13 (D) were examined by in situ hybridization in serial sections at 8 days after grafting. Expressions of fgf-8 (E) and shh (F) were also examined by whole-mount in situ hybridization at 5 days and 8 days after grafting, respectively. G: Lmx-1 expression was examined by in situ hybridization on a section at 8 days after grafting. Arrowheads show the host–graft boundary. Arrows indicate expression domains. a, host anterior side; p, host posterior side; d, host dorsal side; v, host ventral side. Scale bars = 1 mm in A, 250 μm in E,F, 100 μ m in B–D,G. |