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Fig. S3. Transcription factors characterizing the pronephros anlagen are unaffected by depletion of Inversin. (A–L) Unilateral injection of Invs-Mo at the fourcell stage was followed by whole-mount in situ hybridization against transcription factors that characterize the pronephric anlagen, including lim-1 (A and B),
pax-8 (C and D), hepatocyte nuclear factor 1 homeobox B (vHNF1) (E–H), and the tubule-specific transcription factor GATA binding protein 3 (GATA-3) (I–L)
at the indicated stages. No substantial differences were detected compared with the noninjected side. (M) In situ hybridization against Wnt4 visualized in
a 200-μm vibratome section of an embryo. (N) The white arrow points to the in situ signal localized in the somatic layer (so) of the intermediate mesoderm. No
expression was detected in the splanchnic layer (sp) or the endoderm (ed). This indicated correct polarization of the pronephric anlagen. |
