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FIGURE 8. Dnmbp knockdown does not alter expression of early markers of kidney development, as assessed by in situ analysis. Embryos injected unilaterally at the 8-cell stage (blastomere V2) to target the kidney and reared to stage 29–30 for lhx1 expression assessment, stage 32–33 for hnf1β expression assessment and stage 33–34 for pax2 expression assessment. Representative embryo phenotypes shown. Scale bar indicates 500 μm. n = number of embryos across 2 (pax2) or 3 replications (lhx1, hnf1β). Error bars represent Standard error. ∗Significantly different from control, p < 0.05. |