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Fig. 2. Analysis of pdzrn3 depletion effects using pax8 and hnf1b pronephric markers. Mo-pdzrn3 was injected into the marginal zone of one blastomere of Xenopus embryos at 2-cell stage; lacZ mRNA was used as lineage tracer. Control and injected sides of the same embryo are shown, respectively. Stages of embryos are indicated at the bottom right corner of each panel, while the analyzed gene is indicated to left of each row. Abbreviations: otic vesicles (ov), pronephric proximal tubules (pt). (A-F) Impairment of pdzrn3 gene function does affect early expression domains of pax8 and later the morphogenesis of nephrostomes, indicated by arrows. (G-L) In situ hybridization with hnf1b reveals reduction of the pronephron anlagen (black brackets) and pt alterations. The three nephrostomes, clearly detectable in the controls (arrowheads) are indistinct in the morphants. Embryos are shown in lateral views. Scale bars: A,B,G,H, 150 mm; C-F,I-L, 250 mm. |
