|
Gene |
Clone |
Species |
Stages |
Anatomy |
hmga2.L
|
|
laevis
|
NF stage 15
|
neuroectoderm
,
neural crest
,
cardiac mesoderm
|
hmga2.L
|
|
laevis
|
NF stage 23
|
neuroectoderm
,
neural crest
,
tail bud
,
cardiac mesoderm
,
pronephric mesenchyme
,
[+]
|
hmga2.L
|
|
laevis
|
NF stage 32
|
otic vesicle
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
midbrain-hindbrain boundary
,
spinal cord
,
pronephric duct
,
heart
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
branchial arch
,
chordoneural hinge
,
eye
,
cardiac mesoderm
,
lens placode
,
heart primordium
,
tail
,
tail tip
,
primary heart field
,
secondary heart field
|
|
|
Figure 2. (a) Amino-acid sequences of XHMGA2 protein were compared with those of human (NM_001094371), mouse (NM_010441), rat (NM_032070), chicken (NM_205001) and zebrafish (NM_212680) HMGA2. Dots indicate identities and dashed lines are introduced to maximize the alignment. Boxes represent the three AT-hooks. (b) Expression of XHMGA2 mRNA during embryogenesis analysed by RT-PCR. Ornithine decarboxylase (ODC) represents internal controls. (c–h) Expression of XHMGA2 mRNA during embryogenesis analysed by in situ hybridization. Lateral (c) and ventral (d) view of stage-15 embryo. XHMGA2 expression was detected in neural crest (NC) and weakly in precardiac region (arrowhead). Lateral (e) and ventral (f) view of stage-23 embryo. XHMGA2 was expressed in anterior neural tissue (AN), tailbud (TB) and weakly in precardiac region (arrowhead). Lateral (g) and ventral (h) view of stage-32 embryo. XHMGA2 was detected in brain (BR), spinal cord (SC), eye anlage (EA), otic vesicle (OV), branchial arch (BA), pronephric duct (PD) and heart anlage (arrowhead). Anterior is left and dorsal is top for c, e, g, and anterior is left for d, f, h. |