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Experiment details for hmga2

A crucial role of a high mobility group protein HMGA2 in cardiogenesis.

A crucial role of a high mobility group protein HMGA2 in cardiogenesis.

Gene Clone Species Stages Anatomy
hmga2.L laevis NF stage 15 neuroectoderm , neural crest , cardiac mesoderm
hmga2.L laevis NF stage 23 neuroectoderm , neural crest , tail bud , cardiac mesoderm , pronephric mesenchyme , [+]
hmga2.L laevis NF stage 32 otic vesicle , brain , forebrain , midbrain , hindbrain , [+]

  Figure 2. (a) Amino-acid sequences of XHMGA2 protein were compared with those of human (NM_001094371), mouse (NM_010441), rat (NM_032070), chicken (NM_205001) and zebrafish (NM_212680) HMGA2. Dots indicate identities and dashed lines are introduced to maximize the alignment. Boxes represent the three AT-hooks. (b) Expression of XHMGA2 mRNA during embryogenesis analysed by RT-PCR. Ornithine decarboxylase (ODC) represents internal controls. (c–h) Expression of XHMGA2 mRNA during embryogenesis analysed by in situ hybridization. Lateral (c) and ventral (d) view of stage-15 embryo. XHMGA2 expression was detected in neural crest (NC) and weakly in precardiac region (arrowhead). Lateral (e) and ventral (f) view of stage-23 embryo. XHMGA2 was expressed in anterior neural tissue (AN), tailbud (TB) and weakly in precardiac region (arrowhead). Lateral (g) and ventral (h) view of stage-32 embryo. XHMGA2 was detected in brain (BR), spinal cord (SC), eye anlage (EA), otic vesicle (OV), branchial arch (BA), pronephric duct (PD) and heart anlage (arrowhead). Anterior is left and dorsal is top for c, e, g, and anterior is left for d, f, h.