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Experiment details for hhex

Retinoic acid-induced expression of Hnf1b and Fzd4 is required for pancreas development in Xenopus laevis.

Retinoic acid-induced expression of Hnf1b and Fzd4 is required for pancreas development in Xenopus laevis.

Gene Clone Species Stages Anatomy
hhex.L laevis NF stage 35 and 36 to NF stage 37 and 38 liver

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  Fig. S11. Effects of Hnf1β down- or upregulation on the expression of various endodermal organ marker genes Four-cell stage embryos were injected with RNA encoding β-galactosidase and either (A) Hnf1β-morpholino or control-morpholino, or (B) RNA coding for Hnf1β-GR, vegetally into the two dorsal blastomeres. At stage 36/37 embryos were used for WMISH against Pdx1 (pancreas, duodenum), hHex (liver), Nkx2.1 (thyroid, lung) and (B) Darmin (posterior endoderm).

Gene Clone Species Stages Anatomy
hhex.L laevis NF stage 37 and 38 to NF stage 39 liver

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  Fig. S12 Effects of Fzd4/Fzd4s downregulation on the expression of various endodermal organ marker genes Eight-cell stage embryos were injected with RNA coding for β-galactosidase and either Fzd4/ Fzd4s-morpholino or the corresponding mismatch-morpholino. At stage 38/39, embryos were used for WMISH against Pdx1 (pancreas, duodenum), hHex (liver), Nkx2.1 (thyroid, lung) and IFABP (stomach, intestine).