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Figure 1. Injection of G5 MO, but not of C1 and C2 control MOs, cause severe size reduction or loss of heart and liver in Xenopus embryos. A: Translation of injected GATA5-GR.HA mRNA, detected by Western blotting with anti-HA antibody, is blocked by G5 but not by other MOs indicated. G5UTR MO ("G5U") does not affect translation of GATA5-GR.HA, as this constructs lacks the 5'UTR sequence. 1 ng of mRNA was injected into 1- or 2-cell embryos and 105 minutes later the embryos were injected with 10 ng of G5, or 50 ng of G5UTR or G4 MOs. E-Uninjected embryos. B: Injection of 5 ng of G5 MO results in a loss (B1,2) or severe reduction (B3-5) of cardiac and liver precursors, as revealed by whole-mount in situ hybridisation for MLC2 (purple) and Hex (turquoise). Posterior injection of G5 MO (5ng) has no obvious effects (B6). Control MOs 1 or 2 (C1 or C2, 50 ng/embryo) have no effect on normal development of heart and liver precursors (B7-9). B1-4, 8,9: ventral view; B5-7: lateral view (anterior to the left). In B3-5 arrows point to the remnants of the heart. C: G5 MO (1 ng/embryo) causes heart defects in X. tropicalis (Xt) embryos (arrow points to the heart remnant), and C1 MO (10 ng) has no effect on normal heart development (arrowhead in C2). Complete bleaching of X. tropicalis embryos has removed the morphological landmarks, and cement gland (cg) and eyes (e) have been indicated to add visualisation. |
