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Fig. 5. Overexpression of brunol1 results in an overproliferation phenotype. Enlarged pancreas phenotype seen in embryos injected with brunol1 mRNA injected into 2 dorsal-vegetal blastomeres at the eight-cell stage. (A,B) ptf1a expression (n=12). Arrow points to the ectopic pancreas. (C,D) No expression of insulin is detected in the ectopic pancreas (n=24). Pancreas is outlined. (E,F) elastase is expressed in the ectopic pancreatic tissue (arrow, n=23). (G,H) frp5 expression is normal (n=10). Arrow points to overgrowth. (I,J) Expression of the liver marker hex is normal, though the liver does appear a little larger (n=14). (K,L) Trace drawing of panels A and B illustrating the overgrowth of pancreatic tissue in the tadpoles injected with brunol1 mRNA. |
