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Figure 6. Inhibition of akt activities via Wortmannin treatment leads to a phenotype that resembles appl1 knockdown effects. A: Lateral view of embryos that were treated with either DMSO or 100 nM Wortmannin from stage 30 to stage 42. B: Western blot data on embryos treated as described in A. C: TUNEL staining of embryos that were treated with either DMSO or 200 nM Wortmannin from stage 30 to stage 42. 1, 3: Dorsal view. 2, 4: Ventral view. D: Whole mount in situ hybridization data on embryos that were treated either with DMSO or 400 nM Wortmannin from stage 30 to stage 42. 1–6: Lateral view.Download figure to PowerPoint |
