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Fig. 3. Whole mount in situ hybridizations, sequence similarities and functional classifications. The left-most part represents the results of sequence similarity
searches from 273 partially sequenced cDNAs representing unique, differentially expressed genes: clone name; accession number of best hit, P-value; name
of best hit; functional classification. The order of the genes follows first digit sorting, e.g. 1, 10, 2, 20 etc. Abbreviations: o, orphan genes; m, metabolism
gene; r, developmental regulator gene; r?, housekeeping regulator gene; s, structural gene; Xl, known Xenopus gene; +, GRAIL1 good coding potential; ++,
GRAIL1 excellent coding potential. The P-value is the probability provided by the BLAST programme and calculated using Karlinltschul statistics, that
the sequence similarity is due to randomness. For EST hits, the similarities derived from the comparison of the EST or cluster of EST sequences is given. The
right-most part shows in situ hybridizations results for stages 10+, 13 and 30. Abbreviations are: a, animal view (stage 10); a, anterior view (stage 30); d,
dorsal view; l, lateral view; p, posterior view; s, sagittal section; t, transverse section; v, vegetal view (stage 10); v, ventral view (stage 30).
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Fig. 4. Gastrula regionalization. Whole-mount in situ hybridizations of gastrula embryos are shown. Expression in ectodermal and mes-endodermal domains
is shown in upper and lower panel, respectively.(A,D,K) Dorsal view with animal pole facing up; (B,C,G,L), mid-sagittal sections, dorsal facing right, animal
pole facing up; (E,F) animal view, dorsal facing up. (H,I,J) Vegetal view, dorsal facing up. bp, Dorsal blastopore lip; dac, dorsal animal cap; dNIMZ, dorsal
non-involuting marginal zone; eac, epithelial layer of animal cap; sac, sensorial layer of animal cap; vac, ventral animal cap. |
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Fig. 5. Tail regionalization. Whole-mount in situ hybridizations were carried out on tailbud embryos. The tail region in lateral view and vibratome sections
thereof are shown. (A) Show selected marker gene expression, (N and O) show diagrams summarizing the identified domains in mid-sagittal and frontal
view, respectively. Where indicated, frontal (f, posterior to the right), sagittal (s, posterior to the right), or transverse (t, dorsal to the top) vibratome sections
are shown. Abbreviations: acnh, anterior chordoneural hinge; cnh, chordoneural hinge; end, endoderm; fs, forming somites; if, inner fin; mcnh, mid
chordoneural hinge; of, outer fin; nec, neurenteric canal; pag, post-anal gut; pcnh, posterior chordoneural hinge; psm, presomitic mesoderm; pnc, posterior
notochord; pw, posterior wall; scf, spinal cord floor; scr, spinal cord roof; so, somites; tpw, tip of posterior wall; vpw, ventral posterior wall. |
