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Fig. 7. Expression of a dominant negative Su(H) does not affect Hox expression. Non-injected controls (Nic; A,C,E,G) and embryos injected in the left-hand side with dominant negative Su(H) [Su(H)DBM; B,D,F,H] were analysed at neurula stage for the expression of ESR4 (A,B: 85%, n=35) and X-Delta-2 (C,D: 86%, n=37), and at gastrula stage for the expression of Hoxd1 (E,F: 100%, n=21) and Hoxc6 (G,H: 100%, n=23) by whole-mount in situ hybridisation. Embryos at neurula stage are shown from the dorsal side, anterior up, and gastrula embryos from the vegetal side, dorsal up. Su(H)DBM works as a dominant negative affecting the downstream genes of the Notch pathway, ESR4 (B) and inhibiting the X-Delta-2 downregulation in the somitomere region (D) but does not show any effect on Hox expression (F,H). The arrowhead indicates the injected side of the embryo. |
