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Fig. 6. Activation of the Delta-Notch pathway does not affect Hox expression. To activate Notch signalling we overexpressed X-Delta-2 (B,E,H,K,N) or Notch-ICD (C,F,I,L,O) in the LHS of the embryos and analysed them at neurula stage for the expression of ESR4 (A–C), at gastrula stage for the expression of Hoxd1 (D–F) and Hoxc6 (G–I) and at tailbud stage for Hoxb4 (J–L) and Hoxc6 (M–O) expression. Embryos at neurula and tailbud stage are shown from the dorsal side, anterior up; embryos at gastrula stage from the vegetal side, dorsal up. The activation of the Notch pathway, by overexpressing either X-Delta-2 or Notch-ICD perturbs the expression of ESR4 (B: 97%, n=35; C: 95%, n=44), but does not affect the expression of either Hoxd1 (E: 90%, n=40; F: 100%, n=23) or Hoxc6 (H: 100%, n=20; I: 100%, n=21) at gastrula stage, or of Hoxb4 (K: 96%, n=26; L: 90%, n=22) nor Hoxc6 (N: 100%, n=29; O: 90%, n=20) at tailbud stage. The arrowhead indicates the injected side of the embryo. |
