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Fig. 1. Isolation of a bowline upstream region, and reconstitution of bowline expression in transgenic embryos. (A) In situ hybridization for endogenous bowline. (B, C) In situ hybridization for EGFP reporter gene in transgenic embryos with δ3UTRGFP (B), or with FullGFP (C). (D–F) Magnified views of panels A (D), B (E), and C (F). (D′–F′) Schematic diagrams of gene expression in panels D (D′), E (E′), and F (F′). (G) In situ hybridization for endogenous Xhairy2a (h, brown). (H) Double in situ hybridization for Xhairy2a (h, brown) and bowline (b, blue) in wild-type embryos. (I) Double in situ hybridization for Xhairy2a (h, brown) and EGFP reporter gene (g, blue) in transgenic embryos with FullGFP. (G′–I′) Schematic diagrams of gene expression in panels G (G′), H (H′), and I (I′). Arrowheads indicate segmental patterns of expression. The bar indicates the broad expression pattern in transgenic embryos with δ3UTRGFP. |
